› 1q48

Solution NMR

Solution NMR Structure of The Haemophilus Influenzae Iron-Sulfur Cluster Assembly Protein U (IscU) with Zinc Bound at the Active Site. Northeast Structural Genomics Consortium Target IR24. This protein is not apo, it is a model without zinc binding constraints.

Released:
DOI: 10.2210/pdb1q48/pdb
PDB entry 1q48 coloured by chain, ensemble of 20 models, front view.
PDB entry 1q48 coloured by chain, ensemble of 20 models, side view.
PDB entry 1q48 coloured by chain, ensemble of 20 models, top view.
Source organism: Haemophilus influenzae
Primary publication:
Solution NMR structure of the iron-sulfur cluster assembly protein U (IscU) with zinc bound at the active site.
Ramelot TA, Cort JR, Goldsmith-Fischman S, Kornhaber GJ, Xiao R, Shastry R, Acton TB, Honig B, Montelione GT, Kennedy MA
J Mol Biol 344 567-83 (2004)
PMID: 15522305

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176401 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron-sulfur cluster assembly scaffold protein IscU Chain: A
Molecule details ›
Chain: A
Length: 134 amino acids
Theoretical weight: 14.49 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57074 (Residues: 1-126; Coverage: 100%)
Gene names: HI_0377, iscU
Sequence domains: NifU-like N terminal domain
Structure domains: Sufe protein. Chain: A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: SIMULATED ANNEALING, TORSION ANGLE DYNAMICS, AUTOMATED ANALYSIS OF NOESY DATA AND 3D STRUCTURES
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Maturation of iron-sulfur proteins in eukaryotes: mechanisms, connected processes, and diseases.
Lill et al. (2008)
18 more

7 mentions without citation

Hybrid Methods in Iron-Sulfur Cluster Biogenesis.
Prischi et al. (2017)
6 more