1q3v

X-ray diffraction
2.91Å resolution

Crystal structure of a wild-type Cre recombinase-loxP synapse: phosphotyrosine covalent intermediate

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Recombinase cre Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 347 amino acids
Theoretical weight: 39.07 KDa
Source organism: Escherichia virus P1
Expression system: Escherichia coli
UniProt:
  • Canonical: P06956 (Residues: 1-343; Coverage: 100%)
Gene name: cre
Sequence domains: Phage integrase family
Structure domains:
loxP DNA Chains: C, G
Molecule details ›
Chains: C, G
Length: 16 nucleotides
Theoretical weight: 4.96 KDa
Source organism: Punavirus P1
Expression system: Not provided
loxP DNA Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 21 nucleotides
Theoretical weight: 6.45 KDa
Source organism: Punavirus P1
Expression system: Not provided
loxP DNA Chains: D, H
Molecule details ›
Chains: D, H
Length: 37 nucleotides
Theoretical weight: 11.39 KDa
Source organism: Punavirus P1
Expression system: Not provided

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 108.938Å b: 164.16Å c: 194.759Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.247 0.276
Expression systems:
  • Escherichia coli
  • Not provided