X-ray diffraction
1.5Å resolution

Structure of the yeast Hst2 protein deacetylase in ternary complex with 2'-O-acetyl ADP ribose and histone peptide


Function and Biology Details

Reaction catalysed:
(1a) [protein]-N(6)-acetyl-L-lysine + NAD(+) = [protein]-N(6)-(1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl)-L-lysine + nicotinamide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NAD-dependent protein deacetylase HST2 Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 32.65 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
  • Canonical: P53686 (Residues: 5-293; Coverage: 81%)
Gene names: HST2, LPA2C, YPL015C
Sequence domains: Sir2 family
Structure domains:
Histone H4 Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.2 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
  • Canonical: P02309 (Residues: 13-22; Coverage: 10%)
Gene names: HHF1, HHF2, N2752, YBR009C, YBR0122, YNL030W

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: R3
Unit cell:
a: 98.421Å b: 98.421Å c: 77.254Å
α: 90° β: 90° γ: 120°
R R work R free
0.203 0.183 0.21
Expression systems:
  • Escherichia coli
  • Not provided