X-ray diffraction
2.4Å resolution

Carbapenam Synthetase

Source organism: Pectobacterium carotovorum
Primary publication:
Crystal structure of carbapenam synthetase (CarA).
J. Biol. Chem. 278 40996-1002 (2003)
PMID: 12890666

Function and Biology Details

Reaction catalysed:
ATP + (2S,5S)-5-carboxymethylproline = AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Carbapenam-3-carboxylate synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 503 amino acids
Theoretical weight: 56.05 KDa
Source organism: Pectobacterium carotovorum
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9XB61 (Residues: 1-503; Coverage: 100%)
Gene name: carA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 103.042Å b: 61.951Å c: 175.87Å
α: 90° β: 97.01° γ: 90°
R R work R free
0.22 0.22 0.273
Expression system: Escherichia coli BL21(DE3)