› 1q07

X-ray diffraction
2.5Å resolution

Crystal structure of the Au(I) form of E. coli CueR, a copper efflux regulator

Released:
DOI: 10.2210/pdb1q07/pdb
PDB 1q07 coloured by chain and viewed from the front.
PDB 1q07 coloured by chain and viewed from the side.
PDB 1q07 coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Molecular basis of metal-ion selectivity and zeptomolar sensitivity by CueR.
Changela A, Chen K, Xue Y, Holschen J, Outten CE, O'Halloran TV, Mondragón A
Science 301 1383-7 (2003)
PMID: 12958362

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator CueR Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 15.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A9G4 (Residues: 1-135; Coverage: 100%)
Gene names: JW0476, b0487, copR, cueR, ybbI
Sequence domains:
Structure domains: Multidrug-efflux Transporter Regulator; Chain: A; Domain 2

Ligands and Environments

1 bound ligand:
Ligand AU 2 x AU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P212121
Unit cell:
a: 59.265Å b: 66.323Å c: 82.467Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.253
Expression system: Escherichia coli BL21(DE3)

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Citations

66 review citations

Allosteric control of metal-responsive transcriptional regulators in bacteria.
Baksh et al. (2020)
65 more

2 mentions without citation

Removal of distal protein-water hydrogen bonds in a plant epoxide hydrolase increases catalytic turnover but decreases thermostability.
Thomaeus et al. (2008)
1 more