1pyt

X-ray diffraction
2.35Å resolution

TERNARY COMPLEX OF PROCARBOXYPEPTIDASE A, PROPROTEINASE E, AND CHYMOTRYPSINOGEN C

Released:

Function and Biology Details

Reactions catalysed:
Release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.
Preferential cleavage: Leu-|-, Tyr-|-, Phe-|-, Met-|-, Trp-|-, Gln-|-, Asn-|-.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Carboxypeptidase A1 Chain: A
Molecule details ›
Chain: A
Length: 94 amino acids
Theoretical weight: 10.8 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00730 (Residues: 17-110; Coverage: 23%)
Gene names: CPA, CPA1
Sequence domains: Carboxypeptidase activation peptide
Structure domains: Metallocarboxypeptidase-like
Carboxypeptidase A1 Chain: B
Molecule details ›
Chain: B
Length: 309 amino acids
Theoretical weight: 34.79 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00730 (Residues: 111-419; Coverage: 77%)
Gene names: CPA, CPA1
Sequence domains: Zinc carboxypeptidase
Structure domains: Zn peptidases
Proproteinase E Chain: C
Molecule details ›
Chain: C
Length: 253 amino acids
Theoretical weight: 27.34 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P05805 (Residues: 1-253; Coverage: 100%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Chymotrypsin-C Chain: D
Molecule details ›
Chain: D
Length: 251 amino acids
Theoretical weight: 27.39 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q7M3E1 (Residues: 17-268; Coverage: 100%)
Gene name: CTRC
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: R3
Unit cell:
a: 188.5Å b: 188.5Å c: 82.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.192 0.283