1py1

X-ray diffraction
2.6Å resolution

Complex of GGA1-VHS domain and beta-secretase C-terminal phosphopeptide

Released:

Function and Biology Details

Reaction catalysed:
Broad endopeptidase specificity. Cleaves Glu-Val-Asn-Leu-|-Asp-Ala-Glu-Phe in the Swedish variant of Alzheimer's amyloid precursor protein.
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
Non-polymer only tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ADP-ribosylation factor-binding protein GGA1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 158 amino acids
Theoretical weight: 17.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UJY5 (Residues: 2-157; Coverage: 24%)
Gene name: GGA1
Sequence domains: VHS domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Beta-secretase 1 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 8 amino acids
Theoretical weight: 955 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P56817 (Residues: 494-501; Coverage: 2%)
Gene names: BACE, BACE1, KIAA1149

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21212
Unit cell:
a: 70.14Å b: 95.42Å c: 107.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.236 0.288
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided