› 1px5

X-ray diffraction
1.74Å resolution

Crystal structure of the 2'-specific and double-stranded RNA-activated interferon-induced antiviral protein 2'-5'-oligoadenylate synthetase

Released:
DOI: 10.2210/pdb1px5/pdb
PDB 1px5 coloured by chain and viewed from the front.
PDB 1px5 coloured by chain and viewed from the side.
PDB 1px5 coloured by chain and viewed from the top.
Source organism: Sus scrofa
Primary publication:
Crystal structure of the 2'-specific and double-stranded RNA-activated interferon-induced antiviral protein 2'-5'-oligoadenylate synthetase.
Hartmann R, Justesen J, Sarkar SN, Sen GC, Yee VC
Mol. Cell 12 1173-85 (2003)
PMID: 14636576

Function and Biology Details

Reaction catalysed: 
3 ATP = pppA2'p5'A2'p5'A + 2 diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2'-5'-oligoadenylate synthase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 40.42 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q29599 (Residues: 1-349; Coverage: null%)
Gene name: OAS1
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand YCM 4 x YCM

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 46.524Å b: 132.601Å c: 57.305Å
α: 90° β: 99.19° γ: 90°
R-values:
R R work R free
0.187 0.187 0.224
Expression system: Escherichia coli BL21(DE3)

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Citations

21 review citations

New advances in our understanding of the "unique" RNase L in host pathogen interaction and immune signaling.
Gusho et al. (2020)
20 more

16 mentions without citation

RNA-specific ribonucleotidyl transferases.
Martin et al. (2007)
15 more