1pvo

X-ray diffraction
3Å resolution

X-ray crystal structure of Rho transcription termination factor in complex with ssRNA substrate and ANPPNP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Transcription termination factor Rho Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 419 amino acids
Theoretical weight: 47.07 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG30 (Residues: 1-419; Coverage: 100%)
Gene names: JW3756, b3783, nitA, psuA, rho, rnsC, sbaA, tsu
Sequence domains:
Structure domains:
5'-R(P*UP*C)-3' Chains: G, H, J, K, L
Molecule details ›
Chains: G, H, J, K, L
Length: 2 nucleotides
Theoretical weight: 566 Da
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 119.249Å b: 204.649Å c: 147.788Å
α: 90° β: 96.54° γ: 90°
R-values:
R R work R free
0.274 0.271 0.304
Expression systems:
  • Escherichia coli
  • Not provided