› 1pux

Solution NMR

NMR Solution Structure of BeF3-Activated Spo0F, 20 conformers

Released:
DOI: 10.2210/pdb1pux/pdb
PDB entry 1pux coloured by chain, ensemble of 20 models, front view.
PDB entry 1pux coloured by chain, ensemble of 20 models, side view.
PDB entry 1pux coloured by chain, ensemble of 20 models, top view.
Source organism: Bacillus subtilis
Primary publication:
The NMR solution structure of BeF(3)(-)-activated Spo0F reveals the conformational switch in a phosphorelay system.
Gardino AK, Volkman BF, Cho HS, Lee SY, Wemmer DE, Kern D
J Mol Biol 331 245-54 (2003)
PMID: 12875849

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139109 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sporulation initiation phosphotransferase F Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 14.24 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06628 (Residues: 1-124; Coverage: 100%)
Gene names: BSU37130, spo0F
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: Torsion angle dynamics and simulated annealing
Expression system: Escherichia coli

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Citations

1 review citation

Molecular insights into the initiation of sporulation in Gram-positive bacteria: new technologies for an old phenomenon.
Stephenson et al. (2005)
28 other articles

4 mentions without citation

Molecular Mechanisms of Two-Component Signal Transduction.
Zschiedrich et al. (2016)
3 more