› 1po3

X-ray diffraction
3.4Å resolution

Crystal structure of ferric citrate transporter FecA in complex with ferric citrate

Released:
DOI: 10.2210/pdb1po3/pdb
PDB 1po3 coloured by chain and viewed from the front.
PDB 1po3 coloured by chain and viewed from the side.
PDB 1po3 coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Structural evidence for iron-free citrate and ferric citrate binding to the TonB-dependent outer membrane transporter FecA.
Yue WW, Grizot S, Buchanan SK
J. Mol. Biol. 332 353-68 (2003)
PMID: 12948487

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe(3+) dicitrate transport protein FecA Chains: A, B
Molecule details ›
Chains: A, B
Length: 751 amino acids
Theoretical weight: 83.17 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P13036 (Residues: 34-774; Coverage: 100%)
Gene names: JW4251, b4291, fecA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FLC 4 x FLC
Ligand FE 4 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P21
Unit cell:
a: 85.489Å b: 147.003Å c: 96.131Å
α: 90° β: 110.58° γ: 90°
R-values:
R R work R free
0.283 0.239 0.314
Expression system: Escherichia coli BL21

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Citations

25 review citations

Metal-responsive RNA polymerase extracytoplasmic function (ECF) sigma factors.
Moraleda-Muñoz et al. (2019)
24 more