1pn8

Electron Microscopy
10.8Å resolution

Coordinates of S12, L11 proteins and E-site tRNA from 70S crystal structure separately fitted into the Cryo-EM map of E.coli 70S.EF-G.GDPNP complex. The atomic coordinates originally from the E-site tRNA were fitted in the position of the hybrid P/E-site tRNA.

Released:
Primary publication:
Locking and unlocking of ribosomal motions.
Cell 114 123-34 (2003)
PMID: 12859903
Related structures: EMD-1363

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
30S ribosomal protein S12 Chain: O
Molecule details ›
Chain: O
Length: 124 amino acids
Theoretical weight: 13.8 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SHN3 (Residues: 2-125; Coverage: 94%)
Gene names: TTHA1697, rpsL
Sequence domains: Ribosomal protein S12/S23
50S ribosomal protein L11 Chain: L
Molecule details ›
Chain: L
Length: 133 amino acids
Theoretical weight: 14.29 KDa
Source organism: Thermotoga maritima
UniProt:
  • Canonical: P29395 (Residues: 8-140; Coverage: 94%)
Gene names: TM_0454, rplK
Sequence domains: Ribosomal protein L11, RNA binding domain
E-tRNA Chain: D
Molecule details ›
Chain: D
Length: 68 nucleotides
Theoretical weight: 21.84 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 10.8Å
Relevant EMDB volumes: EMD-1363