› 1piw

X-ray diffraction
3Å resolution

APO AND HOLO STRUCTURES OF AN NADP(H)-DEPENDENT CINNAMYL ALCOHOL DEHYDROGENASE FROM SACCHAROMYCES CEREVISIAE

Released:
DOI: 10.2210/pdb1piw/pdb
PDB 1piw coloured by chain and viewed from the front.
PDB 1piw coloured by chain and viewed from the side.
PDB 1piw coloured by chain and viewed from the top.
Source organism: Saccharomyces cerevisiae
Primary publication:
Apo and Holo structures of an NADPH-dependent cinnamyl alcohol dehydrogenase from Saccharomyces cerevisiae.
Valencia E, Larroy C, Ochoa WF, Parés X, Fita I, Biosca JA
J. Mol. Biol. 341 1049-62 (2004)
PMID: 15289102

Function and Biology Details

Reaction catalysed: 
An alcohol + NADP(+) = an aldehyde + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent alcohol dehydrogenase 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 360 amino acids
Theoretical weight: 39.67 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q04894 (Residues: 1-360; Coverage: 100%)
Gene names: ADH6, YM9924.10C, YMR318C
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 234.98Å b: 100.35Å c: 52.49Å
α: 90° β: 92.18° γ: 90°
R-values:
R R work R free
0.266 0.218 0.271
Expression system: Saccharomyces cerevisiae

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Citations

1 review citation

Functions of aldehyde reductases from Saccharomyces cerevisiae in detoxification of aldehyde inhibitors and their biotechnological applications.
Wang et al. (2018)
20 other articles

5 mentions without citation

Prediction and experimental validation of enzyme substrate specificity in protein structures.
Amin et al. (2013)
4 more