1ph2

X-ray diffraction
3.1Å resolution

CRYSTAL STRUCTURE OF THE OXYTRICHA NOVA TELOMERE END-BINDING PROTEIN COMPLEXED WITH NONCOGNATE SSDNA GGGGTTTTG

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Telomere-binding protein subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 459 amino acids
Theoretical weight: 52.3 KDa
Source organism: Sterkiella nova
Expression system: Escherichia coli
UniProt:
  • Canonical: P29549 (Residues: 36-494; Coverage: 93%)
Gene names: MAC-56A, MAC-56K, MAC-56S
Sequence domains: Telomeric single stranded DNA binding POT1/CDC13
Structure domains: Nucleic acid-binding proteins
Telomere-binding protein subunit beta Chain: B
Molecule details ›
Chain: B
Length: 216 amino acids
Theoretical weight: 24.49 KDa
Source organism: Sterkiella nova
Expression system: Escherichia coli
UniProt:
  • Canonical: P16458 (Residues: 9-224; Coverage: 56%)
Gene names: MAC-41A, MAC-41S
Structure domains: Telomere-binding Protein Beta Subunit; Chain
5'-D(*GP*GP*GP*GP*TP*TP*TP*TP*GP*GP*GP*GP*T)-3' Chains: G, H
Molecule details ›
Chains: G, H
Length: 13 nucleotides
Theoretical weight: 4.11 KDa
5'-D(*GP*GP*GP*GP*TP*TP*TP*TP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 9 nucleotides
Theoretical weight: 2.82 KDa

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P6122
Unit cell:
a: 93.65Å b: 93.65Å c: 423.038Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.217 0.269
Expression system: Escherichia coli