› 1pg2

X-ray diffraction
1.75Å resolution

METHIONYL-TRNA SYNTHETASE FROM ESCHERICHIA COLI COMPLEXED WITH METHIONINE AND ADENOSINE

Released:
DOI: 10.2210/pdb1pg2/pdb
PDB 1pg2 coloured by chain and viewed from the front.
PDB 1pg2 coloured by chain and viewed from the side.
PDB 1pg2 coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Use of analogues of methionine and methionyl adenylate to sample conformational changes during catalysis in Escherichia coli methionyl-tRNA synthetase.
Crepin T, Schmitt E, Mechulam Y, Sampson PB, Vaughan MD, Honek JF, Blanquet S
J. Mol. Biol. 332 59-72 (2003)
PMID: 12946347

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 551 amino acids
Theoretical weight: 62.96 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00959 (Residues: 2-552; Coverage: 81%)
Gene names: JW2101, b2114, metG
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADN 1 x ADN
Ligand MET 1 x MET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 77.787Å b: 45.742Å c: 87.51Å
α: 90° β: 108.48° γ: 90°
R-values:
R R work R free
0.201 0.201 0.226
Expression system: Escherichia coli

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Citations

4 review citations

Non-canonical Amino Acid Substrates of E. coli Aminoacyl-tRNA Synthetases.
Hartman MCT. (2022)
3 more

2 mentions without citation

Prospects of In vivo Incorporation of Non-canonical Amino Acids for the Chemical Diversification of Antimicrobial Peptides.
Baumann et al. (2017)
1 more