1pdo

X-ray diffraction
1.7Å resolution

PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM

Released:

Function and Biology Details

Reaction catalysed:
[Protein]-N(pi)-phospho-L-histidine + D-mannose(Side 1) = [protein]-L-histidine + D-mannose 6-phosphate(Side 2)

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS system mannose-specific EIIAB component Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 14.62 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P69797 (Residues: 2-133; Coverage: 41%)
Gene names: JW1806, b1817, gptB, manX, ptsL
Sequence domains: PTS system fructose IIA component
Structure domains: Phosphotransferase system, mannose-type IIA component

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P6122
Unit cell:
a: 76.36Å b: 76.36Å c: 88.73Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.189 0.244
Expression system: Escherichia coli K-12