› 1pc3

X-ray diffraction
2.16Å resolution

Crystal structure of the extracellular phosphate ABC transport receptor (PstS-1) and immunodominant antigen of M. tuberculosis.

Released:
DOI: 10.2210/pdb1pc3/pdb
PDB entry 1pc3 coloured by chain, ensemble of 2 models, front view.
PDB entry 1pc3 coloured by chain, ensemble of 2 models, side view.
PDB entry 1pc3 coloured by chain, ensemble of 2 models, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Crystal structure of M tuberculosis ABC phosphate transport receptor: specificity and charge compensation dominated by ion-dipole interactions.
Vyas NK, Vyas MN, Quiocho FA
Structure 11 765-74 (2003)
PMID: 12842040

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161516 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphate-binding protein PstS 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 35.82 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P9WGU1 (Residues: 25-374; Coverage: 100%)
Gene names: MTCY08D9.05c, Rv0934, phoS1, pstS1
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 125.4Å b: 72.3Å c: 73.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.264
Expression system: Escherichia coli K-12

Quick links

Citations

9 review citations

Structure, function, and evolution of bacterial ATP-binding cassette systems.
Davidson et al. (2008)
8 more

9 mentions without citation

A structural overview of mycobacterial adhesins: Key biomarkers for diagnostics and therapeutics.
Squeglia et al. (2018)
8 more