X-ray diffraction
1.9Å resolution

Structure of human dCK complexed with gemcitabine and ADP-MG

Source organism: Mus musculus

Function and Biology Details

Reactions catalysed:
ATP + deoxyadenosine = ADP + dAMP
ATP + deoxyguanosine = ADP + dGMP
NTP + deoxycytidine = NDP + dCMP
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Deoxycytidine kinase Chain: B
Molecule details ›
Chain: B
Length: 263 amino acids
Theoretical weight: 30.83 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P27707 (Residues: 1-260; Coverage: 100%)
Gene name: DCK
Sequence domains: Deoxynucleoside kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43212
Unit cell:
a: 81.2Å b: 81.2Å c: 94.6Å
α: 90° β: 90° γ: 90°
R R work R free
0.182 0.179 0.207
Expression system: Escherichia coli BL21(DE3)