1p59

X-ray diffraction
2.5Å resolution

Structure of a non-covalent Endonuclease III-DNA Complex

Released:
Primary publication:
Structure of a trapped endonuclease III-DNA covalent intermediate.
EMBO J. 22 3461-71 (2003)
PMID: 12840008

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Endonuclease III Chain: A
Molecule details ›
Chain: A
Length: 226 amino acids
Theoretical weight: 26.24 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
Structure domains:
5'-D(*AP*AP*GP*AP*CP*GP*(5IU)P*GP*GP*AP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.52 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
5'-D(TP*GP*(5IU)P*CP*CP*AP*(3DR)P*GP*(5IU)P*CP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.41 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21212
Unit cell:
a: 66.321Å b: 105.896Å c: 41.518Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.269
Expression systems:
  • Escherichia coli
  • Not provided