Function and Biology

CRYSTAL STRUCTURE OF THE GLYCOSYLASPARAGINASE PRECURSOR D151N MUTANT

Source organism: Elizabethkingia meningoseptica
Biochemical function: hydrolase activity
Biological process: proteolysis
Cellular component: periplasmic space

EC 3.5.1.26: N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase

Reaction catalysed:
N(4)-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H(2)O = N-acetyl-beta-D-glucosaminylamine + L-aspartate
Systematic name:
N(4)-(beta-N-acetyl-D-glucosaminyl)-L-asparagine amidohydrolase
Alternative Name(s):
  • 4-N-(beta-N-acetyl-D-glucosaminyl)-L-asparagine amidohydrolase
  • Aspartylglucosaminidase
  • Aspartylglucosylaminase
  • Aspartylglucosylamine deaspartylase
  • Aspartylglucosylaminidase
  • Aspartylglycosylamine amidohydrolase
  • Beta-aspartylglucosylamine amidohydrolase
  • Glucosylamidase
  • Glycosylasparaginase
  • N-aspartyl-beta-glucosaminidase

Sequence family

Pfam Protein family (Pfam)
PF01112
Domain description: Asparaginase
Occurring in:
  1. N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase
1 copy of Pfam domain PF01112 (Asparaginase) in N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase in PDB 1p4k.

InterPro InterPro annotations
IPR000246
Domain description: Peptidase T2, asparaginase 2
Occurring in:
  1. N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase
IPR029055
Domain description: Nucleophile aminohydrolases, N-terminal
Occurring in:
  1. N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase

Structure domain

SCOP SCOP annotation
56261
Class: Alpha and beta proteins (a+b)
Fold: Ntn hydrolase-like
Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases)
Occurring in:
  1. N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase
1 copy of SCOP domain 56261 ((Glycosyl)asparaginase) in N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase in PDB 1p4k.