1p3w

X-ray diffraction
2.1Å resolution

X-ray crystal structure of E. coli IscS

Released:
Source organism: Escherichia coli
Primary publication:
Crystal structure of IscS, a cysteine desulfurase from Escherichia coli.
J. Mol. Biol. 330 1049-59 (2003)
PMID: 12860127

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase IscS Chains: A, B
Molecule details ›
Chains: A, B
Length: 404 amino acids
Theoretical weight: 45.15 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6B7 (Residues: 1-404; Coverage: 100%)
Gene names: JW2514, b2530, iscS, nuvC, yfhO, yzzO
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 73.709Å b: 101.974Å c: 108.617Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.236
Expression system: Escherichia coli