› 1p1u

X-ray diffraction
2Å resolution

Crystal structure of the GluR2 ligand-binding core (S1S2J) L650T mutant in complex with AMPA (ammonium sulfate crystal form)

Released:
DOI: 10.2210/pdb1p1u/pdb
PDB 1p1u coloured by chain and viewed from the front.
PDB 1p1u coloured by chain and viewed from the side.
PDB 1p1u coloured by chain and viewed from the top.
Source organism: Rattus norvegicus
Primary publication:
Tuning activation of the AMPA-sensitive GluR2 ion channel by genetic adjustment of agonist-induced conformational changes.
Armstrong N, Mayer M, Gouaux E
Proc Natl Acad Sci U S A 100 5736-41 (2003)
PMID: 12730367

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 263 amino acids
Theoretical weight: 29.21 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P19491 (Residues: 413-527, 653-796; Coverage: 30%)
Gene names: Glur2, Gria2
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand AMQ 2 x AMQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 61.434Å b: 96.379Å c: 95.965Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.238 0.271
Expression system: Escherichia coli BL21

Quick links

Citations

20 review citations

Structural Dynamics of Glutamate Signaling Systems by smFRET.
Durham et al. (2020)
19 more

2 mentions without citation

Tuning activation of the AMPA-sensitive GluR2 ion channel by genetic adjustment of agonist-induced conformational changes.
Armstrong et al. (2003)
1 more