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The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactors

Source organism: Klebsiella pneumoniae
Biochemical function: flavin adenine dinucleotide binding
Biological process: butanediol metabolic process
Cellular component: acetolactate synthase complex

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EC 2.2.1.6: Acetolactate synthase

Reaction catalysed:
2 pyruvate = 2-acetolactate + CO(2)
Systematic name:
Pyruvate:pyruvate acetaldehydetransferase (decarboxylating)
Alternative Name(s):
  • Acetohydroxy acid synthetase
  • Acetohydroxyacid synthase
  • Acetolactate pyruvate-lyase (carboxylating)
  • Acetolactic synthetase
  • Alpha-acetohydroxy acid synthetase
  • Alpha-acetohydroxyacid synthase
  • Alpha-acetolactate synthase
  • Alpha-acetolactate synthetase

GO terms

Sequence families

Pfam Protein families (Pfam)
PF02775
Domain description: Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
Occurring in:
  1. Acetolactate synthase, catabolic
The deposited structure of PDB entry 1ozf contains 2 copies of Pfam domain PF02775 (Thiamine pyrophosphate enzyme, C-terminal TPP binding domain) in Acetolactate synthase, catabolic. Showing 1 copy in chain A.

PF00205
Domain description: Thiamine pyrophosphate enzyme, central domain
Occurring in:
  1. Acetolactate synthase, catabolic
The deposited structure of PDB entry 1ozf contains 2 copies of Pfam domain PF00205 (Thiamine pyrophosphate enzyme, central domain) in Acetolactate synthase, catabolic. Showing 1 copy in chain A.

PF02776
Domain description: Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
Occurring in:
  1. Acetolactate synthase, catabolic
The deposited structure of PDB entry 1ozf contains 2 copies of Pfam domain PF02776 (Thiamine pyrophosphate enzyme, N-terminal TPP binding domain) in Acetolactate synthase, catabolic. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR029061
Domain description: Thiamin diphosphate-binding fold
Occurring in:
  1. Acetolactate synthase, catabolic
IPR000399
Domain description: TPP-binding enzyme, conserved site
Occurring in:
  1. Acetolactate synthase, catabolic
IPR011766
Domain description: Thiamine pyrophosphate enzyme, TPP-binding
Occurring in:
  1. Acetolactate synthase, catabolic
IPR012000
Domain description: Thiamine pyrophosphate enzyme, central domain
Occurring in:
  1. Acetolactate synthase, catabolic
IPR029035
Domain description: DHS-like NAD/FAD-binding domain superfamily
Occurring in:
  1. Acetolactate synthase, catabolic
IPR012782
Domain description: Acetolactate synthase, catabolic
Occurring in:
  1. Acetolactate synthase, catabolic
IPR012001
Domain description: Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain
Occurring in:
  1. Acetolactate synthase, catabolic
IPR045229
Domain description: Thiamine pyrophosphate enzyme
Occurring in:
  1. Acetolactate synthase, catabolic

Structure domains

CATH CATH domains
3.40.50.970
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: Rossmann fold
Occurring in:
  1. Acetolactate synthase, catabolic
The deposited structure of PDB entry 1ozf contains 4 copies of CATH domain 3.40.50.970 (Rossmann fold) in Acetolactate synthase, catabolic. Showing 2 copies in chain A.
3.40.50.1220
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: TPP-binding domain
Occurring in:
  1. Acetolactate synthase, catabolic
The deposited structure of PDB entry 1ozf contains 2 copies of CATH domain 3.40.50.1220 (Rossmann fold) in Acetolactate synthase, catabolic. Showing 1 copy in chain A.
SCOP SCOP annotations
88749
Class: Alpha and beta proteins (a/b)
Fold: Thiamin diphosphate-binding fold (THDP-binding)
Superfamily: Thiamin diphosphate-binding fold (THDP-binding)
Occurring in:
  1. Acetolactate synthase, catabolic
The deposited structure of PDB entry 1ozf contains 2 copies of SCOP domain 88749 (Pyruvate oxidase and decarboxylase PP module) in Acetolactate synthase, catabolic. Showing 1 copy in chain A.
52475
Class: Alpha and beta proteins (a/b)
Fold: DHS-like NAD/FAD-binding domain
Superfamily: DHS-like NAD/FAD-binding domain
Occurring in:
  1. Acetolactate synthase, catabolic
The deposited structure of PDB entry 1ozf contains 2 copies of SCOP domain 52475 (Pyruvate oxidase and decarboxylase, middle domain) in Acetolactate synthase, catabolic. Showing 1 copy in chain A.
88724
Class: Alpha and beta proteins (a/b)
Fold: Thiamin diphosphate-binding fold (THDP-binding)
Superfamily: Thiamin diphosphate-binding fold (THDP-binding)
Occurring in:
  1. Acetolactate synthase, catabolic
The deposited structure of PDB entry 1ozf contains 2 copies of SCOP domain 88724 (Pyruvate oxidase and decarboxylase Pyr module) in Acetolactate synthase, catabolic. Showing 1 copy in chain A.

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