› 1own

X-ray diffraction
2.3Å resolution

DATA3:DNA photolyase / received X-rays dose 4.8 exp15 photons/mm2

Released:
DOI: 10.2210/pdb1own/pdb
PDB entry 1own coloured by chain, front view.
PDB entry 1own coloured by chain, side view.
PDB entry 1own coloured by chain, top view.
Source organism: Synechococcus elongatus PCC 6301
Primary publication:
DNA apophotolyase from Anacystis nidulans: 1.8 A structure, 8-HDF reconstitution and X-ray-induced FAD reduction.
Kort R, Komori H, Adachi S, Miki K, Eker A
Acta Crystallogr D Biol Crystallogr 60 1205-13 (2004)
PMID: 15213381

Function and Biology Details

Reaction catalysed: 
Cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138630 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxyribodipyrimidine photo-lyase Chain: A
Molecule details ›
Chain: A
Length: 484 amino acids
Theoretical weight: 54.53 KDa
Source organism: Synechococcus elongatus PCC 6301
Expression system: Escherichia coli
UniProt:
  • Canonical: P05327 (Residues: 1-484; Coverage: 100%)
Gene names: phr, phrA, syc1392_c
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P43212
Unit cell:
a: 89.329Å b: 89.329Å c: 133.403Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.264
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structural biology of DNA photolyases and cryptochromes.
Müller et al. (2009)
1 more