1owf

X-ray diffraction
1.95Å resolution

Crystal structure of a mutant IHF (BetaE44A) complexed with the native H' Site

Released:
Source organism: Escherichia coli
Primary publication:
Integration host factor: putting a twist on protein-DNA recognition.
J. Mol. Biol. 330 493-502 (2003)
PMID: 12842466

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
2 distinct polypeptide molecules
3 distinct DNA molecules
Macromolecules (5 distinct):
Integration host factor subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 99 amino acids
Theoretical weight: 11.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6X7 (Residues: 1-99; Coverage: 100%)
Gene names: JW1702, b1712, hid, himA, ihfA
Sequence domains: Bacterial DNA-binding protein
Structure domains: IHF-like DNA-binding proteins
Integration host factor subunit beta Chain: B
Molecule details ›
Chain: B
Length: 94 amino acids
Theoretical weight: 10.61 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6Y1 (Residues: 1-94; Coverage: 100%)
Gene names: JW0895, b0912, himD, hip, ihfB
Sequence domains: Bacterial DNA-binding protein
Structure domains: IHF-like DNA-binding proteins
Phage lambda H' site Chain: C
Molecule details ›
Chain: C
Length: 35 nucleotides
Theoretical weight: 10.8 KDa
5'-D(*GP*GP*CP*CP*AP*AP*AP*AP*AP*AP*GP*CP*AP*TP*T)-3' Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.61 KDa
5'-D(*GP*CP*TP*TP*AP*TP*CP*AP*AP*TP*TP*TP*GP*TP*TP*GP*CP*AP*CP*C)-3' Chain: E
Molecule details ›
Chain: E
Length: 20 nucleotides
Theoretical weight: 6.07 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 46.66Å b: 58.832Å c: 181.314Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.273
Expression system: Escherichia coli