1otr

Solution NMR

Solution Structure of a CUE-Ubiquitin Complex

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin-binding protein CUE2 Chain: A
Molecule details ›
Chain: A
Length: 49 amino acids
Theoretical weight: 5.57 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P36075 (Residues: 6-54; Coverage: 11%)
Gene names: CUE2, YKL090W
Sequence domains: CUE domain
Structure domains: DNA helicase RuvA subunit, C-terminal domain
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 76 amino acids
Theoretical weight: 8.57 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG63 (Residues: 305-380; Coverage: 20%)
Gene names: SCD2, UBI4, YLL039C
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics and simulated annealing
Expression system: Escherichia coli BL21(DE3)