1ote

X-ray diffraction
1.4Å resolution

E46Q MUTANT OF PHOTOACTIVE YELLOW PROTEIN, P65 AT 110K

Released:
Source organism: Halorhodospira halophila
Primary publication:
Short hydrogen bonds in photoactive yellow protein.
Acta Crystallogr. D Biol. Crystallogr. 60 1008-16 (2004)
PMID: 15159559

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Photoactive yellow protein Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 13.89 KDa
Source organism: Halorhodospira halophila
Expression system: Escherichia coli
UniProt:
  • Canonical: P16113 (Residues: 1-125; Coverage: 100%)
Gene name: pyp
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P65
Unit cell:
a: 40.018Å b: 40.018Å c: 117.61Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.182 0.228
Expression system: Escherichia coli