› 1ota

X-ray diffraction
1.1Å resolution

E46Q MUTANT OF PHOTOACTIVE YELLOW PROTEIN, P63 AT 295K

Released:
DOI: 10.2210/pdb1ota/pdb
PDB 1ota coloured by chain and viewed from the front.
PDB 1ota coloured by chain and viewed from the side.
PDB 1ota coloured by chain and viewed from the top.
Source organism: Halorhodospira halophila
Primary publication:
Short hydrogen bonds in photoactive yellow protein.
Anderson S, Crosson S, Moffat K
Acta Crystallogr. D Biol. Crystallogr. 60 1008-16 (2004)
PMID: 15159559

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Photoactive yellow protein Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 13.89 KDa
Source organism: Halorhodospira halophila
Expression system: Escherichia coli
UniProt:
  • Canonical: P16113 (Residues: 1-125; Coverage: 100%)
Gene name: pyp
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand HC4 1 x HC4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P63
Unit cell:
a: 66.917Å b: 66.917Å c: 41.002Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.153 0.151 0.18
Expression system: Escherichia coli

Quick links

Citations

6 review citations

A short history of structure based research on the photocycle of photoactive yellow protein.
Schmidt M. (2017)
5 more

3 mentions without citation

Combined probes of X-ray scattering and optical spectroscopy reveal how global conformational change is temporally and spatially linked to local structural perturbation in photoactive yellow protein.
Kim et al. (2016)
2 more