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A 1.7A structure of Fve, a member of the new fungal immunomodulatory protein family.

Paaventhan P, Joseph JS, Seow SV, Vaday S, Robinson H, Chua KY, Kolatkar PR
J Mol Biol 332 461-70 (2003)
Cited: 51 times
EuropePMC logo PMID: 12948495

Abstract

Fve, a major fruiting body protein from Flammulina velutipes, a mushroom possessing immunomodulatory activity, stimulates lymphocyte mitogenesis, suppresses systemic anaphylaxis reactions and edema, enhances transcription of IL-2, IFN-gamma and TNF-alpha, and hemagglutinates red blood cells. It appears to be a lectin with specificity for complex cell-surface carbohydrates. Fve is a non-covalently linked homodimer containing no Cys, His or Met residues. It shares sequence similarity only to the other fungal immunomodulatory proteins (FIPs) LZ-8, Gts, Vvo and Vvl, all of unknown structure. The 1.7A structure of Fve solved by single anomalous diffraction of NaBr-soaked crystals is novel: each monomer consists of an N-terminal alpha-helix followed by a fibronectin III (FNIII) fold. The FNIII fold is the first instance of "pseudo-h-type" topology, a transition between the seven beta-stranded s-type and the eight beta-stranded h-type topologies. The structure suggests that dimerization, critical for the activity of FIPs, occurs by 3-D domain swapping of the N-terminal helices and is stabilized predominantly by hydrophobic interactions. The structure of Fve is the first in this lectin family to be reported, and the first of an FNIII domain-containing protein of fungal origin.

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  1. Current Understanding of the Structure and Function of Fungal Immunomodulatory Proteins. Liu Y, Bastiaan-Net S, Wichers HJ. Front Nutr 7 132 (2020)

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  1. Linking in domain-swapped protein dimers. Baiesi M, Orlandini E, Trovato A, Seno F. Sci Rep 6 33872 (2016)
  2. Characterisation of a New Fungal Immunomodulatory Protein from Tiger Milk mushroom, Lignosus rhinocerotis. Pushparajah V, Fatima A, Chong CH, Gambule TZ, Chan CJ, Ng ST, Tan CS, Fung SY, Lee SS, Tan NH, Lim RL. Sci Rep 6 30010 (2016)
  3. Identification and Characterisation of a Novel Protein FIP-sch3 from Stachybotrys chartarum. Li S, Zhao L, Xu W, Jiang Z, Kang J, Wang F, Xin F. PLoS ONE 11 e0168436 (2016)
  4. Identification of a Novel Anti-cancer Protein, FIP-bbo, from Botryobasidium botryosum and Protein Structure Analysis using Molecular Dynamic Simulation. Wang Y, Gao YN, Bai R, Chen HY, Wu YY, Shang JJ, Bao DP. Sci Rep 9 5818 (2019)
  5. Protective Function of Novel Fungal Immunomodulatory Proteins Fip-lti1 and Fip-lti2 from Lentinus tigrinus in Concanavalin A-Induced Liver Oxidative Injury. Gao Y, Wáng Y, Wāng Y, Wu Y, Chen H, Yang R, Bao D. Oxid Med Cell Longev 2019 3139689 (2019)
  6. Synthesis and Antioxidant Studies of 2,4-Dioxothiazolidine-5-acetic Acid Based Organic Salts: SC-XRD and DFT Approach. Mashhadi SMA, Bhatti MH, Jabeen E, Yunus U, Ashfaq M, Akhtar M, Tahir MN, Alshehri SM, Ahmed S, Ojha SC. ACS Omega 8 30186-30198 (2023)


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  1. Coiled coils: attractive protein folding motifs for the fabrication of self-assembled, responsive and bioactive materials. Apostolovic B, Danial M, Klok HA. Chem Soc Rev 39 3541-3575 (2010)
  2. Bioactive proteins from mushrooms. Xu X, Yan H, Chen J, Zhang X. Biotechnol. Adv. 29 667-674 (2011)
  3. Immunomodulation by food: promising concept for mitigating allergic disease? Wichers H. Anal Bioanal Chem 395 37-45 (2009)
  4. Recent status and prospects of the fungal immunomodulatory protein family. Li QZ, Wang XF, Zhou XW. Crit. Rev. Biotechnol. 31 365-375 (2011)
  5. Entomotoxic and nematotoxic lectins and protease inhibitors from fungal fruiting bodies. Sabotič J, Ohm RA, Künzler M. Appl. Microbiol. Biotechnol. 100 91-111 (2016)
  6. Harnessing self-assembled peptide nanoparticles in epitope vaccine design. Negahdaripour M, Golkar N, Hajighahramani N, Kianpour S, Nezafat N, Ghasemi Y. Biotechnol. Adv. 35 575-596 (2017)
  7. Natural Bioactive Compounds from Fungi as Potential Candidates for Protease Inhibitors and Immunomodulators to Apply for Coronaviruses. Suwannarach N, Kumla J, Sujarit K, Pattananandecha T, Saenjum C, Lumyong S. Molecules 25 (2020)
  8. Opportunistic invasive fungal pathogen Macrophomina phaseolina prognosis from immunocompromised humans to potential mitogenic RBL with an exceptional and novel antitumor and cytotoxic effect. Arora P, Dilbaghi N, Chaudhury A. Eur. J. Clin. Microbiol. Infect. Dis. 31 101-107 (2012)
  9. Ganoderma immunomodulatory proteins: mushrooming functional FIPs. Lin J, Chen H, Bai Y, Li S, Liang G, Fan T, Gao N, Wu X, Li H, Chen G, Gao Y, Fan J. Appl Microbiol Biotechnol 106 2367-2380 (2022)

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  1. Beta-propeller crystal structure of Psathyrella velutina lectin: an integrin-like fungal protein interacting with monosaccharides and calcium. Cioci G, Mitchell EP, Chazalet V, Debray H, Oscarson S, Lahmann M, Gautier C, Breton C, Perez S, Imberty A. J. Mol. Biol. 357 1575-1591 (2006)
  2. Structural basis of high-affinity glycan recognition by bacterial and fungal lectins. Imberty A, Mitchell EP, Wimmerová M. Curr. Opin. Struct. Biol. 15 525-534 (2005)
  3. Structural biology sheds light on the puzzle of genomic ORFans. Siew N, Fischer D. J. Mol. Biol. 342 369-373 (2004)
  4. Immunomodulatory capacity of fungal proteins on the cytokine production of human peripheral blood mononuclear cells. Jeurink PV, Noguera CL, Savelkoul HF, Wichers HJ. Int. Immunopharmacol. 8 1124-1133 (2008)
  5. Structural basis for the carbohydrate recognition of the Sclerotium rolfsii lectin. Leonidas DD, Swamy BM, Hatzopoulos GN, Gonchigar SJ, Chachadi VB, Inamdar SR, Zographos SE, Oikonomakos NG. J. Mol. Biol. 368 1145-1161 (2007)
  6. A novel adjuvant Ling Zhi-8 enhances the efficacy of DNA cancer vaccine by activating dendritic cells. Lin CC, Yu YL, Shih CC, Liu KJ, Ou KL, Hong LZ, Chen JD, Chu CL. Cancer Immunol. Immunother. 60 1019-1027 (2011)
  7. Induction of premature senescence in human lung cancer by fungal immunomodulatory protein from Ganoderma tsugae. Liao CH, Hsiao YM, Lin CH, Yeh CS, Wang JC, Ni CH, Hsu CP, Ko JL. Food Chem. Toxicol. 46 1851-1859 (2008)
  8. The genome of the Tiger Milk mushroom, Lignosus rhinocerotis, provides insights into the genetic basis of its medicinal properties. Yap HY, Chooi YH, Firdaus-Raih M, Fung SY, Ng ST, Tan CS, Tan NH. BMC Genomics 15 635 (2014)
  9. Crystal structure of LZ-8 from the medicinal fungus Ganoderma lucidium. Huang L, Sun F, Liang C, He YX, Bao R, Liu L, Zhou CZ. Proteins 75 524-527 (2009)
  10. Cytokines expression induced by Ganoderma sinensis fungal immunomodulatory proteins (FIP-gsi) in mouse spleen cells. Li Q, Wang X, Chen Y, Lin J, Zhou X. Appl. Biochem. Biotechnol. 162 1403-1413 (2010)
  11. Eosinophil apoptosis induced by fungal immunomodulatory peptide-fve via reducing IL-5alpha receptor. Hsieh CW, Lan JL, Meng Q, Cheng YW, Huang HM, Tsai JJ. J. Formos. Med. Assoc. 106 36-43 (2007)
  12. Extracellular expression of a functional recombinant Ganoderma lucidium immunomodulatory protein by Bacillus subtilis and Lactococcus lactis. Yeh CM, Yeh CK, Hsu XY, Luo QM, Lin MY. Appl. Environ. Microbiol. 74 1039-1049 (2008)
  13. Coadministration of the fungal immunomodulatory protein FIP-Fve and a tumour-associated antigen enhanced antitumour immunity. Ding Y, Seow SV, Huang CH, Liew LM, Lim YC, Kuo IC, Chua KY. Immunology 128 e881-94 (2009)
  14. The Sclerotinia sclerotiorum agglutinin represents a novel family of fungal lectins remotely related to the Clostridium botulinum non-toxin haemagglutinin HA33/A. Van Damme EJ, Nakamura-Tsuruta S, Hirabayashi J, Rougé P, Peumans WJ. Glycoconj. J. 24 143-156 (2007)
  15. X-ray sequence ambiguities of Sclerotium rolfsii lectin resolved by mass spectrometry. Sathisha GJ, Prakash YK, Chachadi VB, Nagaraja NN, Inamdar SR, Leonidas DD, Savithri HS, Swamy BM. Amino Acids 35 309-320 (2008)
  16. A new alpha-galactosyl-binding protein from the mushroom Lyophyllum decastes. Goldstein IJ, Winter HC, Aurandt J, Confer L, Adamson JT, Hakansson K, Remmer H. Arch. Biochem. Biophys. 467 268-274 (2007)
  17. Domain 4 of the anthrax protective antigen maintains structure and binding to the host receptor CMG2 at low pH. Williams AS, Lovell S, Anbanandam A, El-Chami R, Bann JG. Protein Sci. 18 2277-2286 (2009)
  18. Genome-based proteomic analysis of Lignosus rhinocerotis (Cooke) Ryvarden sclerotium. Yap HY, Fung SY, Ng ST, Tan CS, Tan NH. Int J Med Sci 12 23-31 (2015)
  19. Recombinant expression of a novel fungal immunomodulatory protein with human tumor cell antiproliferative activity from Nectria haematococca. Li S, Nie Y, Ding Y, Shi L, Tang X. Int J Mol Sci 15 17751-17764 (2014)
  20. Characterization of an immunomodulatory Der p 2-FIP-fve fusion protein produced in transformed rice suspension cell culture. Su CF, Kuo IC, Chen PW, Huang CH, Seow SV, Chua KY, Yu SM. Transgenic Res. 21 177-192 (2012)
  21. Functional expression of FIP-fve, a fungal immunomodulatory protein from the edible mushroom Flammulina velutipes in Pichia pastoris GS115. Lin JW, Jia J, Shen YH, Zhong M, Chen LJ, Chen LJ, Li HG, Ma H, Guo ZF, Qi MF, Liu LX, Li TL. J. Biotechnol. 168 527-533 (2013)
  22. Identification and functional characterization of a novel fungal immunomodulatory protein from Postia placenta. Li SY, Shi LJ, Ding Y, Nie Y, Tang XM. Food Chem. Toxicol. 78 64-70 (2015)
  23. High-Yield Production in Escherichia coli of Fungal Immunomodulatory Protein Isolated from Flammulina velutipes and Its Bioactivity Assay in Vivo. Kong X, Zhang J, Han X, Zhang P, Dai X, Liu J, Zhang X, Lee I, Liu S. Int J Mol Sci 14 2230-2241 (2013)
  24. Characterization of a new fungal immunomodulatory protein, FIP-dsq2 from Dichomitus squalens. Li S, Jiang Z, Sun L, Liu X, Huang Y, Wang F, Xin F. J. Biotechnol. 246 45-51 (2017)
  25. Alleviation of Dermatophagoides microceras-induced allergy by an immunomodulatory protein, FIP-fve, from Flammulina velutipes in mice. Chang YC, Hsiao YM, Hung SC, Chen YW, Ou CC, Chang WT, Lue KH, Ko JL. Biosci. Biotechnol. Biochem. 79 88-96 (2015)
  26. Combination of on-line desalting and HPLC-UV-ESI-MS for simultaneous detection and identification of FIP-fve and flammutoxin in Flammulina velutipes. Tung CH, Lin CC, Tung CC, Chen SF, Sheu F, Lu TJ. J Food Drug Anal 26 1045-1053 (2018)
  27. FIP-sch2, a new fungal immunomodulatory protein from Stachybotrys chlorohalonata, suppresses proliferation and migration in lung cancer cells. Li S, Jiang Z, Xu W, Xie Y, Zhao L, Tang X, Wang F, Xin F. Appl. Microbiol. Biotechnol. 101 3227-3235 (2017)
  28. Identification and Functional Characterization of a Novel Immunomodulatory Protein From Morchella conica SH. Wu G, Sun Y, Deng T, Song L, Li P, Zeng H, Tang X. Front Immunol 11 559770 (2020)
  29. Looking for the X Factor in Bacterial Pathogenesis: Association of orfX-p47 Gene Clusters with Toxin Genes in Clostridial and Non-Clostridial Bacterial Species. Nowakowska MB, Douillard FP, Lindström M. Toxins (Basel) 12 (2019)
  30. Safety assessment of the fungal immunomodulatory protein from Ganoderma microsporum (GMI) derived from engineered Pichia pastoris: Genetic toxicology, a 13-week oral gavage toxicity study, and an embryo-fetal developmental toxicity study in Sprague-Dawley rats. Fu HY, Hseu RS. Toxicol Rep 9 1240-1254 (2022)
  31. Characterization, Recombinant Production, and Bioactivity of a Novel Immunomodulatory Protein from Hypsizygus marmoreus. Yu S, Wang Y, Wu Y, Bao D, Bing W, Li Y, Chen H. Molecules 28 4796 (2023)
  32. FIP-nha, a fungal immunomodulatory protein from Nectria haematococca, induces apoptosis and autophagy in human gastric cancer cells via blocking the EGFR-mediated STAT3/Akt signaling pathway. Li SY, Hou LZ, Gao YX, Zhang NN, Fan B, Wang F. Food Chem (Oxf) 4 100091 (2022)
  33. Functional evaluation of a recombinant fungal immunomodulatory protein from L. rhinocerus produced in P. pastoris and E. coli host expression systems. Ejike UC, Chan CJ, Lim CSY, Lim RLH. Appl Microbiol Biotechnol 105 2799-2813 (2021)
  34. Immunomodulatory Protein from Nectria haematococca Induces Apoptosis in Lung Cancer Cells via the P53 Pathway. Wang JJ, Wang Y, Hou L, Xin F, Fan B, Lu C, Zhang L, Wang F, Li S. Int J Mol Sci 20 (2019)
  35. Systematic analysis of the lysine succinylome in the model medicinal mushroom Ganoderma lucidum. Wang G, Xu L, Yu H, Gao J, Guo L. BMC Genomics 20 585 (2019)


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