X-ray diffraction
3.2Å resolution

The crystal structure of the iron free (Apo-)form of Stearoyl Acyl Carrier Protein Desaturase from Ricinus Communis (Castor Bean).


Function and Biology Details

Reaction catalysed:
Stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O(2) + 2 H(+) = oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 363 amino acids
Theoretical weight: 41.7 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli
  • Canonical: P22337 (Residues: 34-396; Coverage: 92%)
Sequence domains: Fatty acid desaturase
Structure domains: Ribonucleotide Reductase, subunit A

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P31
Unit cell:
a: 188.033Å b: 188.033Å c: 82.047Å
α: 90° β: 90° γ: 120°
R R work R free
0.229 0.228 0.257
Expression system: Escherichia coli