1oph

X-ray diffraction
2.3Å resolution

NON-COVALENT COMPLEX BETWEEN ALPHA-1-PI-PITTSBURGH AND S195A TRYPSIN

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
Non-polymer only dimer (preferred)
Non-polymer only tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alpha-1-antitrypsin Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 44.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01009 (Residues: 26-418; Coverage: 100%)
  • Best match: P01009-2 (Residues: 26-355)
Gene names: AAT, PI, PRO0684, PRO2209, SERPINA1
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:
Cationic trypsin Chain: B
Molecule details ›
Chain: B
Length: 243 amino acids
Theoretical weight: 25.43 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00760 (Residues: 4-246; Coverage: 100%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 132.23Å b: 62.32Å c: 98.88Å
α: 90° β: 110.32° γ: 90°
R-values:
R R work R free
0.19 0.19 0.229
Expression system: Escherichia coli