1ooa

X-ray diffraction
2.45Å resolution

CRYSTAL STRUCTURE OF NF-kB(p50)2 COMPLEXED TO A HIGH-AFFINITY RNA APTAMER

Released:
Source organism: Mus musculus
Primary publication:
Crystal structure of NF-kappaB (p50)2 complexed to a high-affinity RNA aptamer.
Proc. Natl. Acad. Sci. U.S.A. 100 9268-73 (2003)
PMID: 12886018

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Nuclear factor NF-kappa-B p50 subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 326 amino acids
Theoretical weight: 36.65 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P25799 (Residues: 39-363; Coverage: 34%)
Gene name: Nfkb1
Sequence domains:
Structure domains:
RNA aptamer Chains: C, D
Molecule details ›
Chains: C, D
Length: 29 nucleotides
Theoretical weight: 9.33 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 76.753Å b: 151.059Å c: 95.628Å
α: 90° β: 105.95° γ: 90°
R-values:
R R work R free
0.208 0.208 0.248
Expression systems:
  • Escherichia coli BL21
  • Not provided