PDBe 1oo9

Solution NMR

Orientation in Solution of MMP-3 Catalytic Domain and N-TIMP-1 from Residual Dipolar Couplings

Released:
Source organism: Homo sapiens

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Stromelysin-1 Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 18.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Best match: P08254 (Residues: 100-267)
Gene names: MMP3, STMY1
Metalloproteinase inhibitor 1 Chain: B
Molecule details ›
Chain: B
Length: 126 amino acids
Theoretical weight: 14.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Best match: P01033 (Residues: 24-149)
Gene names: CLGI, TIMP, TIMP1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 33%
Refinement method: RIGID BODY MINIMIZATION FOLLOWED BY RESTRAINED SIMULATED ANNEALING
Chemical shifts: BMR5785  
Expression system: Escherichia coli BL21(DE3)