1onv

Solution NMR

NMR Structure of a Complex Containing the TFIIF Subunit RAP74 and the RNAP II CTD Phosphatase FCP1

Released:

Function and Biology Details

Reaction catalysed:
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
General transcription factor IIF subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 82 amino acids
Theoretical weight: 9.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35269 (Residues: 436-517; Coverage: 16%)
Gene names: GTF2F1, RAP74
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
RNA polymerase II subunit A C-terminal domain phosphatase Chain: B
Molecule details ›
Chain: B
Length: 83 amino acids
Theoretical weight: 9.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y5B0 (Residues: 879-961; Coverage: 9%)
Gene names: CTDP1, FCP1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)