1on0

X-ray diffraction
2.2Å resolution

Crystal Structure of Putative Acetyltransferase (YycN) from Bacillus subtilis, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET SR144

Released:
Source organism: Bacillus subtilis
Entry authors: Forouhar F, Shen J, Kuzin A, Chiang Y, Xiao R, Acton TB, Rost B, Montelione GT, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized N-acetyltransferase YycN Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 158 amino acids
Theoretical weight: 18.67 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O32293 (Residues: 1-156; Coverage: 100%)
Gene names: BSU40290, yycN
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 61.891Å b: 101.594Å c: 62.12Å
α: 90° β: 97° γ: 90°
R-values:
R R work R free
0.331 0.225 0.289
Expression system: Escherichia coli BL21