1om2

Solution NMR

SOLUTION NMR STRUCTURE OF THE MITOCHONDRIAL PROTEIN IMPORT RECEPTOR TOM20 FROM RAT IN A COMPLEX WITH A PRESEQUENCE PEPTIDE DERIVED FROM RAT ALDEHYDE DEHYDROGENASE (ALDH)

Released:

Function and Biology Details

Reaction catalysed:
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
Biochemical function:
  • not assigned
Biological process:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitochondrial import receptor subunit TOM20 homolog Chain: A
Molecule details ›
Chain: A
Length: 95 amino acids
Theoretical weight: 10.46 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q62760 (Residues: 51-145; Coverage: 66%)
Gene name: Tomm20
Structure domains: Mitochondrial outer membrane translocase complex, subunit Tom20 domain
Aldehyde dehydrogenase, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.23 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: P11884 (Residues: 12-22; Coverage: 2%)
Gene name: Aldh2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: TORSION ANGLE DYNAMICS FOLLOWED BY RESTRAINED ENERGY MINIMIZATION WITH AMBER FORCE FIELD
Chemical shifts: BMR4496  
Expression systems:
  • Escherichia coli
  • Not provided