X-ray diffraction
2.5Å resolution

Structure of Aurora-A 122-403, phosphorylated on Thr287, Thr288 and bound to TPX2 1-43

Source organism: Homo sapiens
Primary publication:
Structural basis of Aurora-A activation by TPX2 at the mitotic spindle.
Mol. Cell 12 851-62 (2003)
PMID: 14580337

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aurora kinase A Chain: A
Molecule details ›
Chain: A
Length: 282 amino acids
Theoretical weight: 32.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: O14965 (Residues: 122-403; Coverage: 70%)
Gene names: AIK, AIRK1, ARK1, AURA, AURKA, AYK1, BTAK, IAK1, STK15, STK6
Sequence domains: Protein kinase domain
Structure domains:
Targeting protein for Xklp2 Chain: B
Molecule details ›
Chain: B
Length: 43 amino acids
Theoretical weight: 4.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9ULW0 (Residues: 1-43; Coverage: 6%)
Gene names: C20orf1, C20orf2, DIL2, HCA519, TPX2

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 59.63Å b: 81.72Å c: 83.05Å
α: 90° β: 90° γ: 90°
R R work R free
0.194 0.194 0.252
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)