› 1okg

X-ray diffraction
2.1Å resolution

3-mercaptopyruvate sulfurtransferase from Leishmania major

Released:
DOI: 10.2210/pdb1okg/pdb
PDB 1okg coloured by chain and viewed from the front.
PDB 1okg coloured by chain and viewed from the side.
PDB 1okg coloured by chain and viewed from the top.
Source organism: Leishmania major
Primary publication:
The crystal structure of Leishmania major 3-mercaptopyruvate sulfurtransferase. A three-domain architecture with a serine protease-like triad at the active site.
Alphey MS, Williams RA, Mottram JC, Coombs GH, Hunter WN
J. Biol. Chem. 278 48219-27 (2003)
PMID: 12952945

Function and Biology Details

Reaction catalysed: 
(1a) 2-oxo-3-sulfanylpropanoate + [3-mercaptopyruvate sulfurtransferase]-L-cysteine = pyruvate + [3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-mercaptopyruvate sulfurtransferase Chain: A
Molecule details ›
Chain: A
Length: 373 amino acids
Theoretical weight: 41.3 KDa
Source organism: Leishmania major
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7K9G0 (Residues: 1-370; Coverage: 100%)
Gene names: LMJF_05_0970, MST
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand SO3 1 x SO3
3 modified residues:
Ligand MSE 13 x MSE
Ligand CSR 1 x CSR
Ligand CSS 2 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P42212
Unit cell:
a: 109.574Å b: 109.574Å c: 67.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.208 0.287
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Chemical Biology of H2S Signaling through Persulfidation.
Filipovic et al. (2018)
4 more

1 mention without citation

Identification of recurring protein structure microenvironments and discovery of novel functional sites around CYS residues.
Wu et al. (2010)