1ohp

X-ray diffraction
1.53Å resolution

CRYSTAL STRUCTURE OF 5-3-KETOSTEROID ISOMERASE MUTANT D38N FROM PSEUDOMONAS TESTOSTERONI COMPLEXED WITH 5ALPHA-ESTRAN-3,17-DIONE

Released:
Source organism: Comamonas testosteroni
Entry authors: Byun M, Kim M-S, Oh B-H

Function and Biology Details

Reaction catalysed:
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Steroid Delta-isomerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 125 amino acids
Theoretical weight: 13.42 KDa
Source organism: Comamonas testosteroni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00947 (Residues: 1-125; Coverage: 100%)
Gene name: ksi
Sequence domains: Nuclear transport factor 2 (NTF2) domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6B
Spacegroup: P21
Unit cell:
a: 39.353Å b: 95.352Å c: 60.402Å
α: 90° β: 91.47° γ: 90°
R-values:
R R work R free
0.203 0.203 0.229
Expression system: Escherichia coli BL21(DE3)