1oh5

X-ray diffraction
2.9Å resolution

THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A C:A MISMATCH

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA mismatch repair protein MutS Chains: A, B
Molecule details ›
Chains: A, B
Length: 800 amino acids
Theoretical weight: 89.6 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P23909 (Residues: 1-800; Coverage: 94%)
Gene names: JW2703, b2733, fdv, mutS
Sequence domains:
Structure domains:
5'-D(*AP*GP*CP*TP*GP*CP*CP*AP*CP*GP *CP*AP*CP*CP*AP*GP*TP*GP*TP*CP*AP*GP*CP*GP*TP *CP*CP*TP*AP*T)-3' Chain: E
Molecule details ›
Chain: E
Length: 30 nucleotides
Theoretical weight: 9.14 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*AP*TP*AP*GP*GP*AP*CP*GP*CP*TP *GP*AP*CP*AP*CP*TP*GP*GP*TP*GP*CP*AP*TP*GP*GP *CP*AP*GP*CP*T)-3' Chain: F
Molecule details ›
Chain: F
Length: 30 nucleotides
Theoretical weight: 9.29 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 89.913Å b: 91.887Å c: 261.173Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.224 0.294
Expression systems:
  • Escherichia coli
  • Not provided