› 1of8

X-ray diffraction
1.5Å resolution

double complex of the tyrosine sensitive DAHP Synthase from S. cerevisiae with Co2+, PEP and the E4P analogoue G3P

Released:
DOI: 10.2210/pdb1of8/pdb
PDB entry 1of8 coloured by chain, front view.
PDB entry 1of8 coloured by chain, side view.
PDB entry 1of8 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Substrate and metal complexes of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Saccharomyces cerevisiae provide new insights into the catalytic mechanism.
König V, Pfeil A, Braus GH, Schneider TR
J Mol Biol 337 675-90 (2004)
PMID: 15019786

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152317 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibited Chains: A, B
Molecule details ›
Chains: A, B
Length: 370 amino acids
Theoretical weight: 39.8 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32449 (Residues: 1-370; Coverage: 100%)
Gene names: ARO4, YBR1701, YBR249C
Sequence domains: DAHP synthetase I family
Structure domains: Aldolase class I

Ligands and Environments

4 bound ligands:
Ligand CO 2 x CO
Ligand PEP 2 x PEP
Ligand G3P 2 x G3P
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: C2
Unit cell:
a: 196.462Å b: 50.788Å c: 64.7Å
α: 90° β: 106.39° γ: 90°
R-values:
R R work R free
0.127 0.124 0.167
Expression system: Saccharomyces cerevisiae

Quick links

Citations

2 review citations

The diversity of allosteric controls at the gateway to aromatic amino acid biosynthesis.
Light et al. (2013)
1 more

2 mentions without citation

Neisseria meningitidis expresses a single 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase that is inhibited primarily by phenylalanine.
Cross et al. (2013)
1 more