1oc2

X-ray diffraction
1.5Å resolution

The structure of NADH in the dTDP-D-glucose dehydratase (RmlB) enzyme

Released:
Source organism: Streptococcus suis
Primary publication:
The structure of NADH in the enzyme dTDP-d-glucose dehydratase (RmlB).
J. Am. Chem. Soc. 125 11872-8 (2003)
PMID: 14505409

Function and Biology Details

Reaction catalysed:
dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H(2)O
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dTDP-glucose 4,6-dehydratase Chains: A, B

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 61.41Å b: 94.829Å c: 183.543Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.17 0.2
Expression system: Escherichia coli BL21(DE3)