X-ray diffraction
2Å resolution

crystal structure of the S133A mutant of Malonamidase E2 complexed with malonate from Bradyrhizobium japonicum


Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Amidase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 414 amino acids
Theoretical weight: 43.72 KDa
Source organism: Bradyrhizobium japonicum
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9ZIV5 (Residues: 1-414; Coverage: 100%)
Sequence domains: Amidase
Structure domains: Amidase signature (AS) domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6B
Spacegroup: P21212
Unit cell:
a: 103.629Å b: 95.225Å c: 74.981Å
α: 90° β: 90° γ: 90°
R R work R free
0.188 0.188 0.245
Expression system: Escherichia coli BL21(DE3)