X-ray diffraction
1.7Å resolution

Comparison of Family 12 Glycoside Hydrolases and Recruited Substitutions Important for Thermal Stability


Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Endoglucanase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 218 amino acids
Theoretical weight: 23.49 KDa
Source organism: Trichoderma citrinoviride
Expression system: Aspergillus niger
  • Canonical: Q8NJY6 (Residues: 17-234; Coverage: 100%)
Gene name: cel12A
Sequence domains: Glycosyl hydrolase family 12
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 62.49Å b: 77.547Å c: 83.41Å
α: 90° β: 98.46° γ: 90°
R R work R free
0.193 0.192 0.217
Expression system: Aspergillus niger