1oa2

X-ray diffraction
1.5Å resolution

Comparison of Family 12 Glycoside Hydrolases and Recruited Substitutions Important for Thermal Stability

Released:

Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-1,4-glucanase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 218 amino acids
Theoretical weight: 23.5 KDa
Source organism: Trichoderma reesei
Expression system: Aspergillus niger
UniProt:
  • Canonical: O00095 (Residues: 17-234; Coverage: 100%)
Gene name: egl
Sequence domains: Glycosyl hydrolase family 12
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 68.28Å b: 71.29Å c: 119.3Å
α: 90° β: 91.49° γ: 90°
R-values:
R R work R free
0.206 0.205 0.223
Expression system: Aspergillus niger