1o9t

X-ray diffraction
2.9Å resolution

Methionine adenosyltransferase complexed with both substrates ATP and methionine

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine synthase isoform type-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 396 amino acids
Theoretical weight: 43.64 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13444 (Residues: 1-397; Coverage: 100%)
Gene names: Ams1, Mat1a
Sequence domains:
Structure domains: GMP Synthetase; Chain A, domain 3

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: P4122
Unit cell:
a: 114.99Å b: 114.99Å c: 160.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.265 0.265 0.288
Expression system: Escherichia coli BL21(DE3)