1o9j

X-ray diffraction
2.4Å resolution

The X-ray crystal structure of eta-crystallin

Released:

Function and Biology Details

Reaction catalysed:
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase, cytosolic 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 501 amino acids
Theoretical weight: 54.6 KDa
Source organism: Elephantulus edwardii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q28399 (Residues: 1-501; Coverage: 100%)
Gene name: ALDH1
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P21
Unit cell:
a: 80.869Å b: 136.407Å c: 84.988Å
α: 90° β: 102.62° γ: 90°
R-values:
R R work R free
0.184 0.18 0.254
Expression system: Escherichia coli BL21(DE3)