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Ternary complex between trimethylamine dehydrogenase and electron transferring flavoprotein

X-ray diffraction
3.7Å resolution
PDB entry 1o95 coloured by chain, front view.
PDB entry 1o95 coloured by chain, side view.
PDB entry 1o95 coloured by chain, top view.
Source organism: Methylophilus methylotrophus
Assembly composition:
hetero hexamer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero hexamer
Accessible surface area: 76441.14 Å2
Buried surface area: 27992.05 Å2
Dissociation area: 998.55 Å2
Dissociation energy (ΔGdiss): -13.75 kcal/mol
Dissociation entropy (TΔSdiss): 29.22 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-147610

Macromolecules

Trimethylamine dehydrogenase
Chains: A, B
Length: 729 amino acids
Theoretical weight: 81.61 KDa
Source organism: Methylophilus methylotrophus
UniProt:
  • Canonical: P16099 (Residues: 2-730; Coverage: 100%)
Gene name: tmd
Pfam:
InterPro:
CATH:
SCOP:
Trimethylamine dehydrogenase in PDB entry 1o95, assembly 1, front view.
Trimethylamine dehydrogenase in PDB entry 1o95, assembly 1, side view.
Trimethylamine dehydrogenase in PDB entry 1o95, assembly 1, top view.
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Electron transfer flavoprotein subunit beta
Chains: C, E
Length: 264 amino acids
Theoretical weight: 28.93 KDa
Source organism: Methylophilus methylotrophus
Expression system: Escherichia coli
UniProt:
  • Canonical: P53570 (Residues: 1-264; Coverage: 100%)
Gene name: etfB
Pfam: Electron transfer flavoprotein domain
InterPro:
CATH: HUPs
SCOP: ETFP subunits
Electron transfer flavoprotein subunit beta in PDB entry 1o95, assembly 1, front view.
Electron transfer flavoprotein subunit beta in PDB entry 1o95, assembly 1, side view.
Electron transfer flavoprotein subunit beta in PDB entry 1o95, assembly 1, top view.
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Electron transfer flavoprotein subunit alpha
Chains: D, F
Length: 320 amino acids
Theoretical weight: 33.62 KDa
Source organism: Methylophilus methylotrophus
Expression system: Escherichia coli
UniProt:
  • Canonical: P53571 (Residues: 2-321; Coverage: 100%)
Gene name: etfA
Pfam:
InterPro:
CATH: HUPs
SCOP: ETFP subunits
Electron transfer flavoprotein subunit alpha in PDB entry 1o95, assembly 1, front view.
Electron transfer flavoprotein subunit alpha in PDB entry 1o95, assembly 1, side view.
Electron transfer flavoprotein subunit alpha in PDB entry 1o95, assembly 1, top view.
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