› 1o3r

X-ray diffraction
3Å resolution

PROTEIN-DNA RECOGNITION AND DNA DEFORMATION REVEALED IN CRYSTAL STRUCTURES OF CAP-DNA COMPLEXES

Released:
DOI: 10.2210/pdb1o3r/pdb
PDB 1o3r coloured by chain and viewed from the front.
PDB 1o3r coloured by chain and viewed from the side.
PDB 1o3r coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Indirect readout of DNA sequence at the primary-kink site in the CAP-DNA complex: DNA binding specificity based on energetics of DNA kinking.
Chen S, Vojtechovsky J, Parkinson GN, Ebright RH, Berman HM
J Mol Biol 314 63-74 (2001)
PMID: 11724532

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
cAMP-activated global transcriptional regulator CRP Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 22.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACJ8 (Residues: 9-208; Coverage: 95%)
Gene names: JW5702, b3357, cap, crp, csm
Structure domains:
5'-D(*AP*AP*AP*AP*AP*TP*GP*CP*GP*AP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.39 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*CP*TP*AP*GP*AP*TP*CP*GP*CP*AP*TP*TP*TP*TP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.55 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CMP 2 x CMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3121
Unit cell:
a: 76.9Å b: 76.9Å c: 143.64Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.233 0.233 0.283
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Syn, anti, and finally both conformations of cyclic AMP are involved in the CRP-dependent transcription initiation mechanism in E. coli lac operon.
Tutar Y. (2008)
31 other articles

2 mentions without citation

Catabolite activator protein: DNA binding and transcription activation.
Lawson et al. (2004)
1 more