› 1nzb

X-ray diffraction
3.1Å resolution

Crystal structure of wild type Cre recombinase-loxP synapse

Released:
DOI: 10.2210/pdb1nzb/pdb
PDB 1nzb coloured by chain and viewed from the front.
PDB 1nzb coloured by chain and viewed from the side.
PDB 1nzb coloured by chain and viewed from the top.
Source organism: Escherichia virus P1
Primary publication:
Crystal structure of a wild-type Cre recombinase-loxP synapse reveals a novel spacer conformation suggesting an alternative mechanism for DNA cleavage activation.
Ennifar E, Meyer JE, Buchholz F, Stewart AF, Suck D
Nucleic Acids Res 31 5449-60 (2003)
PMID: 12954782

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Recombinase cre Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 343 amino acids
Theoretical weight: 38.6 KDa
Source organism: Escherichia virus P1
Expression system: Escherichia coli
UniProt:
  • Canonical: P06956 (Residues: 1-343; Coverage: 100%)
Gene name: cre
Sequence domains: Phage integrase family
Structure domains:
loxP DNA Chains: C, G
Molecule details ›
Chains: C, G
Length: 37 nucleotides
Theoretical weight: 11.36 KDa
Source organism: Escherichia virus P1
Expression system: Not provided
loxP DNA Chains: D, H
Molecule details ›
Chains: D, H
Length: 37 nucleotides
Theoretical weight: 11.39 KDa
Source organism: Escherichia virus P1
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 10 x MG
Ligand IOD 2 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 108.89Å b: 163.53Å c: 195.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.257
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Mechanisms of site-specific recombination.
Grindley et al. (2006)
26 other articles

5 mentions without citation

Crystal structure of a wild-type Cre recombinase-loxP synapse reveals a novel spacer conformation suggesting an alternative mechanism for DNA cleavage activation.
Ennifar et al. (2003)
4 more